Hydroxyapatite coated magnesium alloy for peripheral nerve regeneration
Almansoori Akram Abdo, 주경원, 김봉준, 김성민, 이성미, 이종호,
소속 상세정보
( Almansoori Akram Abdo ) - Seoul National University School of Dentistry Department of Oral and Maxillofacial Surgery
주경원 ( Ju Kyung-Won ) - Seoul National University Dental Hospital Clinical Translational Research Center for Dental Science
김봉준 ( Kim Bong-Jun ) - Seoul National University Dental Hospital Clinical Translational Research Center for Dental Science
김성민 ( Kim Soung-Min ) - Seoul National University School of Dentistry Department of Oral and Maxillofacial Surgery
이성미 ( Lee Sung-Mi ) - Seoul National University College of Engineering Department of Material Science and Engineering
이종호 ( Lee Jong-Ho ) - Seoul National University School of Dentistry Department of Oral and Maxillofacial Surgery
Abstract
Magnesium (Mg) has been used as a neuroprotective agent and for orthopedic and cardiovascular stent applications for a long time. Mg is a promising material for neural regeneration own to its metallic nature, biodegradability, and biocompatibility. To evaluate the benefit of hydroxyapatite (HA) coated Mg alloy (WE43) nerve conduit in peripheral nerve injury regeneration. In vitro cell adhesion and proliferation of PC12 cells and Schwann cells over pure Mg, WE43, HA coated pure Mg (HA-Mg), and HA coated WE43 (HA-WE43) disks were assessed using scanning electron microscope and EZ-CYTOX assay. In vivo study was also performed for sham, silicone nerve conduits, and HA-WE43 nerve conduits to evaluate rat sciatic nerve regeneration over 10 mm gap. These rats were observed for three months with weekly functional assessment using sciatic functional index. Regeneration was evaluated through retrograde neuron labeling and histomorphometric analysis at the end of the experiment. Non-coated pure Mg and WE43 conduits showed fast resorption rate and high gas formation. Higher adhesion and proliferation of PC12 cells were found in HA-WE43 disks. At the end of three months, HA-WE43 nerve conduit showed mild resorption without detectable gas formation in the surrounding tissue. However, only scanty regenerated neural tissue was found within the conduit. HA-WE43 nerve conduit showed controlled degradation and absence of gas formation with scanty regenerated neural tissue.
키워드
Hydroxyapatite; Magnesium; Nerve conduit; Peripheral nerve regeneration
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